Do Sterilization Processes Really Make Difference in Dialysis-Induced Genotoxicity?

Emna El Golli-Bennour, Chiraz Zaied, Chayma Bouaziz, Yosra Guedri, Abdellatif Achour, Salwa Abid, Hassen Bacha

Abstract


The major problem associated with hemodialysis (HD) therapy is the choice of dialyzer membrane matters as well as the mode of sterilization required before utilization. In this context, we aimed to determine the effect of two sterilization processes (steam and gamma rays) on genotoxicity of polysulfone dialysis membranes. A 38-year-old man with end-stage renal disease and one age-matched healthy subject were enrolled in our study. We analyzed lipid peroxidation measured by serum malondialdehyde (MDA) formation and DNA fragmentation assessed by agarose gel electrophoresis and diphenylamine quantification. Our results clearly showed that there is a rising generation of MDA and DNA fragmentation in patient sera during HD session when compared to healthy subject data. This increase in MDA and DNA fragmentation amounts followed respectively the sterilization process. As classified from the more biocompatible one, the steam sterilized polysulfone dialyzer is the top ranked, followed by the gamma rays sterilized one. We concluded that using steam instead of gamma rays for sterilization may improve the biocompatibility of polysulfone dialyzer membranes.




World J Nephrol Urol. 2017;6(1-2):14-17
doi: https://doi.org/10.14740/wjnu296e

Keywords


Dialyzer membranes; Sterilization process; Oxidative stress; Genotoxicity

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